Phase-Separated Biomolecular Condensates: Methods and Protocols (Methods in Molecular Biology, 2563)

This volume provides readers with a broad collection of theoretical, computational, and experimental methods to quantitatively study the properties of phase-separate biomolecular condensates in diverse systems. The chapters in this book cover topics such as theoretical and computational methods; methods for in vitro characterization of biomolecular condensates; and techniques that enable in-cell characterization of biomolecular condensates. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.
Comprehensive and thorough,Phase-Separated Biomolecular Condensates: Methods and Protocolsis a valuable resource that helps researchers learn and use established methods to study both biophysical properties and biological functions of biomolecular condensates.